9LDY
Crystallization of putative zinc protease from Porphyromonas gingivalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-01-03 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.50 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 111.926, 87.012, 120.371 |
| Unit cell angles | 90.00, 111.55, 90.00 |
Refinement procedure
| Resolution | 26.510 - 3.100 |
| R-factor | 0.2443 |
| Rwork | 0.242 |
| R-free | 0.29040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.508 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MLPHARE |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.173 | 0.639 |
| Number of reflections | 35447 | 3164 |
| <I/σ(I)> | 14.2 | |
| Completeness [%] | 89.8 | |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 287.15 | 20% PEG 3350, 0.2 M Disodium malonate pH 7.0 |
