9LDN

Verticillium effector PevD1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-09-29
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.987
Spacegroup name	C 2 2 21
Unit cell lengths	58.407, 79.592, 55.281
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.850 - 1.350
R-factor	0.2207
Rwork	0.220
R-free	0.23940
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.948
Data reduction software	autoPX (beta3)
Data scaling software	HKL-3000 (7.21)
Phasing software	PHENIX ((1.19.2_4158: 000))
Refinement software	PHENIX ((1.19.2_4158: 000))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.370
High resolution limit [Å]	1.300	1.330
Number of reflections	32006	2293
<I/σ(I)>	31.34	4.7
Completeness [%]	100.0	100
Redundancy	12.4
CC(1/2)	0.998	0.904

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		289	3.2 M sodium formate