9LDG

The structure of TkoKptA/DNA/Appr>P complex-B

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-01-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.987
Spacegroup name	P 1 21 1
Unit cell lengths	60.078, 59.397, 65.749
Unit cell angles	90.00, 94.04, 90.00

Refinement procedure

Resolution	29.660 - 2.400
R-factor	0.2386
Rwork	0.236
R-free	0.28960
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.663
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.19.1_4122: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.590	2.380
High resolution limit [Å]	2.260	2.260
Rmerge	0.115	0.861
Rmeas	0.142	1.154
Rpim	0.082	0.762
Number of reflections	18988	1718
<I/σ(I)>	4.8	1.1
Completeness [%]	86.7
Redundancy	2.4
CC(1/2)	0.980	0.415

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	300	200 mM Calcium acetate 20% w/v PEG 3350 pH 7.5