9LCW
Grimontia hollisae thermostable direct hemolysin K88A mutant in complex with 1-nt long 3'-overhang dsDNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE TPS 05A |
| Synchrotron site | NSRRC |
| Beamline | TPS 05A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-06-21 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.603, 61.656, 113.283 |
| Unit cell angles | 90.00, 106.06, 90.00 |
Refinement procedure
| Resolution | 29.780 - 2.707 |
| R-factor | 0.25139 |
| Rwork | 0.248 |
| R-free | 0.31679 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.147 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.8.0415) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.780 | 2.740 |
| High resolution limit [Å] | 2.707 | 2.707 |
| Number of reflections | 29284 | 1427 |
| <I/σ(I)> | 11.025 | |
| Completeness [%] | 85.1 | |
| Redundancy | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 200 mM Ammonium sulfate, 100 mM Bis Tris/ Hydrochloric acid pH 5.5 |
