9LCS

Grimontia hollisae thermostable direct hemolysin in complex with 2-nt long 5'-overhang dsDNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 07A
Synchrotron site	NSRRC
Beamline	TPS 07A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-16
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	1
Spacegroup name	P 4 21 2
Unit cell lengths	79.647, 79.647, 74.243
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.700 - 1.578
R-factor	0.18586
Rwork	0.185
R-free	0.19715
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	1.146
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0415)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.701	1.610
High resolution limit [Å]	1.578	1.578
Number of reflections	33502	1634
<I/σ(I)>	41.32
Completeness [%]	100.0
Redundancy	12.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M Lithium sulfate monohydrate, 0.1 M Sodium citrate tribasic dihydrate pH 5.0, 26% v/v Polyethylene glycol 200