9LCM

Grimontia hollisae thermostable direct hemolysin in complex with 6-nt blunt-ended dsDNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-22
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	1.0
Spacegroup name	C 2 2 21
Unit cell lengths	112.844, 122.556, 60.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.670 - 1.820
R-factor	0.16636
Rwork	0.165
R-free	0.19507
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.470
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0415)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.670	1.850
High resolution limit [Å]	1.820	1.820
Number of reflections	37999	1843
<I/σ(I)>	26
Completeness [%]	99.9
Redundancy	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	30% (v/v) MPD, 100 mM Sodium acetate/ Hydrochloric acid pH 4.6, 20 mM Calcium chloride