9LBV

Pseudomonas aeruginosa Histidinol dehydrogenase with ADPP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER X8 PROTEUM
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-05-15
Detector	Bruker PHOTON III
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	67.117, 89.153, 140.875
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.080 - 1.850
R-factor	0.2276
Rwork	0.227
R-free	0.26630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8xsq
RMSD bond length	0.008
RMSD bond angle	0.890
Data reduction software	PROTEUM PLUS
Data scaling software	PROTEUM PLUS
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.650	1.900
High resolution limit [Å]	1.850	1.850
Number of reflections	159588	6125
<I/σ(I)>	1.6
Completeness [%]	99.0
Redundancy	7
CC(1/2)	0.990	0.850

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		295	12% PEG 8000, 100 mM Na-Acetate pH 4.5