9LB2

E2s domain of HEV ORF2 type 4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-21
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 43
Unit cell lengths	53.641, 53.641, 134.398
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.504 - 1.400
R-factor	0.1537
Rwork	0.153
R-free	0.16770
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.202
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.420
High resolution limit [Å]	1.400	1.400
Number of reflections	74407	7425
<I/σ(I)>	59.2
Completeness [%]	99.9
Redundancy	14.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293.15	0.1 M Bis-Tris buffer (pH 5.5) and 2 M (NH4)2SO4