9L9Z

Fab of anti-HEV mAb 6H8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-21
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	69.589, 69.469, 78.734
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.582 - 1.500
R-factor	0.1837
Rwork	0.182
R-free	0.20960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3s62
RMSD bond length	0.003
RMSD bond angle	0.674
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.530
High resolution limit [Å]	1.500	4.070	1.500
Rmerge	0.065	0.045	0.869
Total number of observations	586793
Number of reflections	60969	3084	2733
<I/σ(I)>	13.3
Completeness [%]	98.5	92.4	89.7
Redundancy	9.6	9.1	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293.15	20% PEG1500