9L9Y
Immune complex of HEV E2s and mAb 6H8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | H 3 |
| Unit cell lengths | 196.880, 196.880, 115.923 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.163 - 2.440 |
| R-factor | 0.2171 |
| Rwork | 0.216 |
| R-free | 0.23560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.584 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.480 |
| High resolution limit [Å] | 2.440 | 6.600 | 2.440 |
| Rmerge | 0.070 | 0.048 | 0.486 |
| Total number of observations | 692673 | ||
| Number of reflections | 62284 | 3013 | 3126 |
| <I/σ(I)> | 13.9 | ||
| Completeness [%] | 99.8 | 96.7 | 100 |
| Redundancy | 11.1 | 9.7 | 11.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 293.15 | 0.1 M Tris-HCl buffer (pH 8.5) with 25% w/v polyethylene glycol (PEG) 3350 |
