9L9W

Structure of SPARTA in complex with guide DNA and a 19nt target DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-12-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 32 2 1
Unit cell lengths	136.189, 136.189, 174.919
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.310 - 5.870
R-factor	0.2625
Rwork	0.261
R-free	0.28840
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.520
Data reduction software	HKL-3000 (7.21)
Data scaling software	HKL-3000 (7.21)
Phasing software	PHENIX (1.17.1_3660)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.320	6.020
High resolution limit [Å]	5.870	5.870
Rpim	0.059	0.903
Number of reflections	5233	371
<I/σ(I)>	7.5
Completeness [%]	99.6	100
Redundancy	17.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M BICINE, pH=8.5, 4.4% PEG4000