9L9T
Crystal structure of a coronaviral M protein in complex with a C-terminal peptide of the N protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-05-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 77.544, 220.132, 71.097 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.106 - 3.142 |
| R-factor | 0.2563 |
| Rwork | 0.255 |
| R-free | 0.29090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.474 |
| Data reduction software | XDS (BUILT 20230630) |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.151 | 3.127 |
| High resolution limit [Å] | 3.025 | 3.025 |
| Rmerge | 0.165 | 1.283 |
| Rmeas | 0.185 | 1.407 |
| Rpim | 0.081 | 0.570 |
| Total number of observations | 50696 | 3065 |
| Number of reflections | 10543 | 527 |
| <I/σ(I)> | 7.2 | 1.3 |
| Completeness [%] | 86.0 | |
| Redundancy | 4.8 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 8.8 | 293.15 | 300 mM ammonium formate, 50 mM Tris-HCl pH 8.8, 35% PEG 500 MME, 7.1 mM pentaethylene glycol monooctyl ether, soaked with 1 mM VPSFEDVVDA peptide |
