9L9R

Room-temperature structure of lysozyme determined by serial synchrotron crystallography

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	298.5
Detector technology	PIXEL
Collection date	2019-02-25
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9794
Spacegroup name	P 43 21 2
Unit cell lengths	78.980, 78.980, 38.240
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.850 - 1.450
R-factor	0.1803
Rwork	0.179
R-free	0.19390
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.811
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.000	1.500
High resolution limit [Å]	1.450	1.450
Number of reflections	22185	2153
<I/σ(I)>	9.6
Completeness [%]	100.0
Redundancy	710
CC(1/2)	0.994	0.543

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES		293.5	sodium acetate, NaCl, PEG8000