9L9I

Crystal structure of ARMS 1-4 ARs in complex with GABARAP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-05-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97915
Spacegroup name	P 21 21 21
Unit cell lengths	44.954, 58.310, 92.649
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.420 - 2.000
R-factor	0.207
Rwork	0.205
R-free	0.24010
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.792
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.030
High resolution limit [Å]	2.000	5.420	2.000
Rmerge	0.076	0.058	0.388
Rmeas	0.080	0.061	0.405
Rpim	0.025	0.020	0.117
Total number of observations	201278
Number of reflections	17000	919	832
<I/σ(I)>	9.7
Completeness [%]	98.6	95	99.4
Redundancy	11.8	9.5	11.6
CC(1/2)	0.998	0.998	0.953

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	45% (w/v) PEE, 100mM MES and 400 mM Potassium chloride