9L92
The crystal structure of human RyR3 Repeat12 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 297 |
| Detector technology | PIXEL |
| Collection date | 2024-11-29 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.040, 122.152, 47.711 |
| Unit cell angles | 90.00, 93.27, 90.00 |
Refinement procedure
| Resolution | 47.600 - 1.970 |
| Rwork | 0.228 |
| R-free | 0.27900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.630 | 2.070 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.074 | 0.800 |
| Rmeas | 0.080 | 0.881 |
| Rpim | 0.031 | 0.362 |
| Total number of observations | 213715 | 26843 |
| Number of reflections | 32492 | 4737 |
| <I/σ(I)> | 19.7 | 2.6 |
| Completeness [%] | 99.8 | |
| Redundancy | 6.6 | 5.7 |
| CC(1/2) | 0.999 | 0.768 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 0.1M Bis-Tris pH 6.5, 28% PEG 2000 |
