9L91

The crystal structure of human RyR3 Repeat12 domain in complex with Azumolene and AMP-PCP

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	297
Detector technology	PIXEL
Collection date	2024-10-27
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	261.666, 59.960, 60.759
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	130.830 - 3.000
R-factor	0.2813
Rwork	0.268
R-free	0.31670
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	130.830	3.160
High resolution limit [Å]	3.000	3.000
Rmerge	0.097	0.862
Rmeas	0.086	0.910
Rpim	0.024	0.285
Number of reflections	20109	2894
<I/σ(I)>	13.4
Completeness [%]	96.2
Redundancy	6.7	6
CC(1/2)	0.995	0.901

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	0.4M sodium chloride, 0.1M HEPES pH 7.5, 20% PEG 3350