9L8Z
The complex structure of antibody CF22 bound to the hemagglutinin of influenza B virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-10-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 114.987, 77.163, 121.653 |
| Unit cell angles | 90.00, 93.38, 90.00 |
Refinement procedure
| Resolution | 32.020 - 3.448 |
| Rwork | 0.262 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.020 | 3.572 |
| High resolution limit [Å] | 3.448 | 3.448 |
| Number of reflections | 28323 | 28323 |
| <I/σ(I)> | 7.59 | |
| Completeness [%] | 99.4 | |
| Redundancy | 1.1 | |
| CC(1/2) | 1.000 | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 290 | 1M MES, PH6, 50% (W/V) PEG 8000, 1M Zn(OA)2 |
