9L8R

Dihydroxyacid dehydratase (DHAD)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97877
Spacegroup name	P 42 21 2
Unit cell lengths	136.040, 136.040, 66.829
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.040 - 2.190
R-factor	0.1865
Rwork	0.184
R-free	0.23110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ym0
RMSD bond length	0.008
RMSD bond angle	1.012
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.040	2.260
High resolution limit [Å]	2.190	2.190
Number of reflections	32804	2782
<I/σ(I)>	2.2
Completeness [%]	99.9
Redundancy	13.1
CC(1/2)	0.933	0.791

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.1 M sodium acetate pH 5.0, 1.5 M ammonium sulfate