9L86
Structure of frog TREX1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2022-01-14 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 65.453, 86.090, 95.397 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.180 - 1.400 |
| R-factor | 0.1502 |
| Rwork | 0.150 |
| R-free | 0.15880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7tqn |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.980 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.21.1_5127) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.180 | 1.440 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rpim | 0.022 | 0.192 |
| Number of reflections | 51237 | 3793 |
| <I/σ(I)> | 31.1 | 3.2 |
| Completeness [%] | 96.4 | 72.5 |
| Redundancy | 11.1 | 4.7 |
| CC(1/2) | 0.996 | 0.875 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.75 M (NH4)2SO4, 0.1 M Bis-Tris pH 5.5, 1.5% PEG3350 |
