9L7L

Structure of the MATE family multidrug resistance transporter HvAACT1 from Hordeum vulgare in the outward-facing state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 43 21 2
Unit cell lengths	96.450, 96.450, 206.280
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.910 - 3.210
R-factor	0.2622
Rwork	0.260
R-free	0.30040
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.666
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.390
High resolution limit [Å]	3.200	3.200
Rmerge	0.077
Number of reflections	16789	2647
<I/σ(I)>	11.45
Completeness [%]	99.8
Redundancy	7.7
CC(1/2)	0.998	0.543

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		281	PEG 300, Na-citrate