9L6K
Crystal structure of nucleotide-free human kinesin-1 motor domain (G234V mutant)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.120, 162.370, 53.200 |
| Unit cell angles | 90.00, 114.50, 90.00 |
Refinement procedure
| Resolution | 19.490 - 2.800 |
| R-factor | 0.2033 |
| Rwork | 0.200 |
| R-free | 0.25820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.539 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.500 | 2.870 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.051 | 0.081 |
| Number of reflections | 16383 | 1011 |
| <I/σ(I)> | 29.58 | 13.25 |
| Completeness [%] | 97.6 | 85.6 |
| Redundancy | 7.14 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 277 | 20% PEG 3350, 100mM HEPES-NaOH, 200mM ammonium acetate, 3% xylitol |
