9L6B

A ROS-Sensing Transcription Factor Promotes RpoS Accumulation to Resist Oxidative Stress

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-01-02
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.987
Spacegroup name	P 43 21 2
Unit cell lengths	58.937, 58.937, 124.367
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.470 - 2.300
R-factor	0.2309
Rwork	0.229
R-free	0.27120
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.088
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.260	29.469
High resolution limit [Å]	2.300	2.303
Rmerge	0.131
Rmeas	0.132
Rpim	0.020	0.019
Number of reflections	10353	10294
<I/σ(I)>	18	17.8
Completeness [%]	92.9	98.9
Redundancy	3.7
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	1.5 M Ammonium sulfate, 0.1 M BIS-TRIS propane pH 7.0