9L5U
Papain-like cysteine protease toxin/immunity pair
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE TPS 05A |
| Synchrotron site | NSRRC |
| Beamline | TPS 05A |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2022-03-29 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.99984 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 104.137, 104.137, 128.709 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.490 - 1.870 |
| R-factor | 0.1722 |
| Rwork | 0.171 |
| R-free | 0.19710 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.968 |
| Data reduction software | HKL-2000 (v7.22) |
| Phasing software | PHENIX (1.19.2_4158) |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.490 | 1.940 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Number of reflections | 85800 | 5359 |
| <I/σ(I)> | 28.9 | |
| Completeness [%] | 98.9 | |
| Redundancy | 9.6 | |
| CC(1/2) | 1.000 | 0.935 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | magnesium formate, sodium acetate |
