9L55

Plastid Localized Exonuclease 1 (D249A) complexed with DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-07-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979183
Spacegroup name	P 21 21 21
Unit cell lengths	96.450, 104.400, 117.350
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.380 - 2.870
R-factor	0.272
Rwork	0.269
R-free	0.31510
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.101
Data scaling software	xia2
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.380	2.940
High resolution limit [Å]	2.870	2.870
Rmerge	0.139	1.790
Rmeas	0.145
Rpim	0.040	0.647
Number of reflections	51965	26697
<I/σ(I)>	19.3	1.6
Completeness [%]	99.8	100
Redundancy	12.8	13.2
CC(1/2)	0.999	0.739

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	295.15	0.2 M ammonium acetate, 0.01 M MgCl2, 0.05 M Sodium cacodylate pH 6.5, 10% w/v PEG 4000