9L4P

Arabidopsis thaliana protease-associated domain of vacuolar sorting receptor 1 in complexed with vicilin-like seed storage protein 22 C-terminal pentapeptide SDRFV (pH 7.0)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E
Temperature [K]	93
Detector technology	PIXEL
Collection date	2023-05-14
Detector	DECTRIS EIGER R 4M
Wavelength(s)	1.5419
Spacegroup name	P 1 21 1
Unit cell lengths	32.701, 60.435, 39.206
Unit cell angles	90.00, 113.29, 90.00

Refinement procedure

Resolution	30.220 - 1.950
R-factor	0.1773
Rwork	0.174
R-free	0.23670
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.810
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.010	2.000
High resolution limit [Å]	1.950	1.950
Number of reflections	10101	713
<I/σ(I)>	8.4
Completeness [%]	98.1
Redundancy	2.9
CC(1/2)	0.996	0.788

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	289.15	0.1 M MES pH 7.0 25% (w/v) PEG 6000