9L4O

Arabidopsis thaliana protease-associated domain of vacuolar sorting receptor 1 in complexed with vicilin-like seed storage protein 22 C-terminal pentapeptide SDRFV (pH 5.8)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	93
Detector technology	PIXEL
Collection date	2023-05-12
Detector	DECTRIS EIGER R 4M
Wavelength(s)	1.5419
Spacegroup name	P 1 21 1
Unit cell lengths	32.658, 60.384, 39.245
Unit cell angles	90.00, 113.38, 90.00

Refinement procedure

Resolution	30.190 - 1.900
R-factor	0.1708
Rwork	0.169
R-free	0.21570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.807
Data reduction software	XDS (VERSION Jan 10, 2022 BUILT=20220820)
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.21 1-5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.020	1.940
High resolution limit [Å]	1.900	1.900
Number of reflections	10890	2236
<I/σ(I)>	19
Completeness [%]	98.2
Redundancy	3.7
CC(1/2)	0.999	0.885

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.8	289.15	0.1 M MES pH 5.8 30% (w/v) PEG 6000