9L4I

Crystal structure of HLA-C*14:02 complexed with KIR2DL2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	95
Detector technology	CCD
Collection date	2024-05-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97946
Spacegroup name	I 2 2 2
Unit cell lengths	83.950, 160.150, 177.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.050 - 2.500
R-factor	0.231
Rwork	0.230
R-free	0.25330
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.613
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.050	2.589
High resolution limit [Å]	2.500	2.500
Rmerge	0.030	0.196
Rmeas	0.043	0.278
Rpim	0.030	0.196
Number of reflections	41811	4148
<I/σ(I)>	14.89	3.93
Completeness [%]	99.9	99.98
Redundancy	2	2
CC(1/2)	0.998	0.891

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	0.2 M Potassium citrate tribasic monohydrate and 20% w/v Polyethylene glycol 3,350