9L4H

Crystal structure of HLA-C*14:03 complexed with KIR2DL2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	95
Detector technology	CCD
Collection date	2024-05-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97946
Spacegroup name	I 2 2 2
Unit cell lengths	84.110, 162.480, 176.440
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.200 - 3.100
R-factor	0.2186
Rwork	0.215
R-free	0.25170
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.675
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.200	3.211
High resolution limit [Å]	3.100	3.100
Rmerge		0.195
Rmeas		0.275
Rpim		0.195
Number of reflections	22340	2175
<I/σ(I)>	11.76
Completeness [%]	99.9
Redundancy	2
CC(1/2)	0.993	0.883

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	0.2 M Potassium phosphate dibasic and 20% w/v Polyethylene glycol 3,350