9L4A

Crystal structure of HLA-C*12:02-MY9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17UM
Synchrotron site	SSRF
Beamline	BL17UM
Temperature [K]	95
Detector technology	CCD
Collection date	2023-05-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9785
Spacegroup name	P 21 21 21
Unit cell lengths	79.520, 96.400, 121.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.220 - 1.900
R-factor	0.1655
Rwork	0.164
R-free	0.20730
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.019
RMSD bond angle	1.487
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.200	1.940
High resolution limit [Å]	1.900	1.900
Rmerge	0.097	0.502
Rmeas	0.106	0.554
Rpim	0.031	0.232
Number of reflections	73658	4455
<I/σ(I)>	17
Completeness [%]	99.6	98.7
Redundancy	11.2	10.9
CC(1/2)	0.998	0.915

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	0.2M Sodium formate 0.1M Bis-Tris propane pH8.5 20% w/v PEG3,350