9L47
Crystal structure of HLA-C*12:03-MY9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2023-05-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.490, 136.770, 68.920 |
| Unit cell angles | 90.00, 107.77, 90.00 |
Refinement procedure
| Resolution | 44.270 - 2.800 |
| R-factor | 0.2128 |
| Rwork | 0.207 |
| R-free | 0.26460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.444 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.340 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.069 | 0.273 |
| Rmeas | 0.098 | 0.386 |
| Rpim | 0.069 | 0.273 |
| Number of reflections | 18891 | 1857 |
| <I/σ(I)> | 9.88 | |
| Completeness [%] | 93.4 | 92.2 |
| Redundancy | 5.9 | 5.6 |
| CC(1/2) | 0.989 | 0.797 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 0.2 M Lithium sulfate 0.1M Tris pH8.5 30% w/v PEG4,000 |
