9L3L
Crystal structure of the C lobe of E6AP in complex with K48-linked diUb
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-05-22 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.978565 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 61.070, 121.690, 81.320 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.792 - 2.585 |
| R-factor | 0.2222 |
| Rwork | 0.217 |
| R-free | 0.26880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.785 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX (1.13_2998) |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.792 | 2.678 |
| High resolution limit [Å] | 2.585 | 2.585 |
| Rmerge | 0.132 | |
| Rmeas | 0.156 | |
| Rpim | 0.083 | |
| Number of reflections | 9641 | 935 |
| <I/σ(I)> | 6.87 | |
| Completeness [%] | 92.7 | 82.65 |
| Redundancy | 3.3 | 3.4 |
| CC(1/2) | 0.990 | 0.425 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M Sodium citrate tribasic dihydrate, 0.1 M TRIS hydrochloride pH 8.5, 30% v/v Polyethylene glycol 400 |
