Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2024-01-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97861 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 81.260, 133.020, 146.020 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 64.000 - 1.770 |
| R-factor | 0.18129 |
| Rwork | 0.180 |
| R-free | 0.21075 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6m61 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.733 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MR-Rosetta |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.090 | 1.790 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.096 | |
| Number of reflections | 154217 | 5053 |
| <I/σ(I)> | 17.3 | |
| Completeness [%] | 100.0 | |
| Redundancy | 13.1 | |
| CC(1/2) | 0.531 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.1 M zinc acetate dihydrate pH 5.0,0.2 M Sodiumacetate trihydrate, 16% polyethylene glycol 3350. |
