9L35

Crystal structure of C45G9.5 protein from Caenorhabditis elegans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	273
Detector technology	PIXEL
Collection date	2021-07-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97915
Spacegroup name	C 1 2 1
Unit cell lengths	59.475, 76.033, 137.371
Unit cell angles	90.00, 92.90, 90.00

Refinement procedure

Resolution	25.466 - 1.597
R-factor	0.1765
Rwork	0.176
R-free	0.21240
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.016
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.630
High resolution limit [Å]	1.597	4.340	1.600
Rmerge	0.072	0.050	0.464
Rmeas	0.078	0.054	0.502
Rpim	0.029	0.020	0.190
Number of reflections	79896	4094	3950
<I/σ(I)>	7.8
Completeness [%]	99.0	98.8	99.7
Redundancy	6.8	6.8	6.8
CC(1/2)	0.996	0.998	0.955

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7	293	25% PEG 3350, 0.1 M HEPES pH 7.0, 0.2 M (NH4)2SO4