9L2R
Crystal structure of the SeMet-derived C-terminal of viral responsive protein 15 (PmVRP15) from black tiger shrimp Penaeus monodon
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 293.15 |
| Detector technology | PIXEL |
| Collection date | 2020-02-18 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979115 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 66.619, 115.297, 54.935 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.770 - 1.900 |
| R-factor | 0.2082 |
| Rwork | 0.207 |
| R-free | 0.22840 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.768 |
| Data reduction software | XDS (Jun 30, 2024) |
| Data scaling software | XDS (Jun 30, 2024) |
| Phasing software | PHENIX (1.20.1_4487) |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.770 | 1.968 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.102 | 0.861 |
| Rmeas | 0.111 | 0.937 |
| Rpim | 0.043 | 0.365 |
| Number of reflections | 17053 | 1683 |
| <I/σ(I)> | 15.33 | 3.3 |
| Completeness [%] | 99.9 | 99.88 |
| Redundancy | 6.6 | 6.4 |
| CC(1/2) | 0.998 | 0.827 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | Ammonium sulfate, sodium chloride, CHES |
