9L1J
sdAbB YERLA mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-05-17 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 77.929, 77.929, 45.283 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.283 - 1.620 |
| Rwork | 0.160 |
| R-free | 0.19940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.440 |
| Data reduction software | XDS (20230630) |
| Data scaling software | ADDREF (0.7.13) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.300 | 1.650 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Rmerge | 0.072 | 0.898 |
| Rpim | 0.025 | 0.295 |
| Number of reflections | 20480 | 990 |
| <I/σ(I)> | 16.3 | 2.6 |
| Completeness [%] | 100.0 | |
| Redundancy | 9.9 | |
| CC(1/2) | 0.998 | 0.787 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293.15 | 0.1M HEPES Sodium, 2% PEG400, 1.5M Ammonium sulfate |
