9L12
Crystal structure of Cas12h ternary complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-06-08 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979852 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 155.527, 155.527, 479.655 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.420 - 3.810 |
| R-factor | 0.3345 |
| Rwork | 0.334 |
| R-free | 0.33920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | AlphaFold |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.190 |
| Data reduction software | HKL-3000 (7.21) |
| Data scaling software | HKL-3000 (7.21) |
| Phasing software | PHENIX (1.17.1_3660) |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.660 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rpim | 0.115 | 0.449 |
| Number of reflections | 79206 | 3586 |
| <I/σ(I)> | 4 | |
| Completeness [%] | 94.7 | 92.8 |
| Redundancy | 4.9 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 289 | 0.1 M Tris-HCl, 0.2 M Magnesium chloride, 31% PEG 400. |
