9L0V

Structure of WDR5 in complex with WIN motif containing ZSCAN10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-11-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	C 1 2 1
Unit cell lengths	112.395, 46.613, 64.854
Unit cell angles	90.00, 111.94, 90.00

Refinement procedure

Resolution	42.550 - 2.000
R-factor	0.1808
Rwork	0.179
R-free	0.22000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.211
Data reduction software	autoPX
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.197	0.378
Number of reflections	21211	1546
<I/σ(I)>	13.9
Completeness [%]	1.0
Redundancy	6.1
CC(1/2)	0.962	0.906

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M sodium citrate, pH 4.5, and 20 % (w/v) PEG 4000