9L0T

Structure of WDR5 in complex with WIN motif containing RBM15

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 1
Unit cell lengths	46.937, 61.486, 64.716
Unit cell angles	68.92, 89.00, 67.83

Refinement procedure

Resolution	43.060 - 2.050
R-factor	0.2147
Rwork	0.213
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.993
Data reduction software	autoPX
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.100
High resolution limit [Å]	2.050	2.050
Rmerge	0.073	0.319
Number of reflections	38213	2787
<I/σ(I)>	18.1
Completeness [%]	98.4
Redundancy	3.4
CC(1/2)	0.993	0.916

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2 M imidazole malate, pH 6.0, and 15 % (w/v) PEG 4000