9L0Q

The crystal structure of the beta-glucosidase Ks5A7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	CCD
Collection date	2022-06-02
Detector	OXFORD ONYX CCD
Wavelength(s)	1.54184
Spacegroup name	I 1 2 1
Unit cell lengths	94.121, 83.886, 145.551
Unit cell angles	90.00, 90.42, 90.00

Refinement procedure

Resolution	24.230 - 2.400
R-factor	0.1839
Rwork	0.182
R-free	0.21460
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.585
Data reduction software	CrysalisPro
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.230	2.530
High resolution limit [Å]	2.400	2.400
Rmerge	0.190	0.340
Number of reflections	41530	6232
<I/σ(I)>	8.6	2.9
Completeness [%]	94.0	96.9
Redundancy	2.4	2.3
CC(1/2)	0.989	0.840

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	15% PEG 3350, 200 mM NaCl, 100 mM Tris-HCl pH 8.0, 100 mM CaCl2