9KZR

Anti-CRISPR protein AcrIIA7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	125
Detector technology	PIXEL
Collection date	2021-06-02
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97959
Spacegroup name	P 21 21 21
Unit cell lengths	54.790, 77.830, 133.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.320 - 2.160
R-factor	0.2035
Rwork	0.201
R-free	0.25120
Structure solution method	SAD
RMSD bond length	0.024
RMSD bond angle	2.350
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AutoSol
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.320	2.237
High resolution limit [Å]	2.160	2.160
Number of reflections	31408	3071
<I/σ(I)>	12.89
Completeness [%]	99.9
Redundancy	13.1
CC(1/2)	0.997	0.837

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	15% (w/v) PEG 3000, 0.1M acetate pH 4.7