9KYI

Crystal structure of mouse Endothelial-overexpressed lipopolysaccharide-associated factor 1 (EOLA1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-06-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97736
Spacegroup name	P 2 21 21
Unit cell lengths	33.252, 50.943, 96.292
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.850 - 1.550
R-factor	0.1758
Rwork	0.175
R-free	0.19780
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.862
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.21rc1_5156)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.610
High resolution limit [Å]	1.550	1.550
Rmerge	0.081	0.179
Rmeas	0.187	0.187
Rpim	0.017	0.050
Number of reflections	24456	2333
<I/σ(I)>	6.7	2.7
Completeness [%]	99.7	97.2
Redundancy	21.1	11.7
CC(1/2)	0.995	0.986

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M HEPES sodium pH 7.5, 2% v/v Polyethylene glycol 400, 2.0 M Ammonium sulfate