9KYG
Crystal structure of E.coli ac4C amidohydrolase YqfB in complex with cytidine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-10 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 108.439, 30.493, 61.321 |
| Unit cell angles | 90.00, 94.17, 90.00 |
Refinement procedure
| Resolution | 29.350 - 1.470 |
| R-factor | 0.1886 |
| Rwork | 0.187 |
| R-free | 0.21680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.926 |
| Data reduction software | autoPROC |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.550 |
| High resolution limit [Å] | 1.470 | 1.470 |
| Rmerge | 0.078 | 0.540 |
| Rmeas | 0.088 | 0.626 |
| Rpim | 0.039 | 0.309 |
| Number of reflections | 33689 | 4192 |
| <I/σ(I)> | 10.4 | 2.1 |
| Completeness [%] | 97.5 | 84 |
| Redundancy | 4.9 | 3.7 |
| CC(1/2) | 0.997 | 0.843 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M Magnesium chloride, 0.1M Tris-HCl pH 8.5, 30% PEG 4,000 |
