9KYF
Crystal structure of E.coli ac4C amidohydrolase YqfB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-10 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 108.306, 30.550, 61.949 |
| Unit cell angles | 90.00, 93.90, 90.00 |
Refinement procedure
| Resolution | 29.400 - 1.550 |
| R-factor | 0.2035 |
| Rwork | 0.201 |
| R-free | 0.24520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.809 |
| Data reduction software | autoPROC |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21rc1_5156) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.630 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.089 | 0.179 |
| Rmeas | 0.097 | 0.197 |
| Rpim | 0.038 | 0.080 |
| Number of reflections | 29807 | 4293 |
| <I/σ(I)> | 13.4 | 6.7 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.4 | 6 |
| CC(1/2) | 0.994 | 0.979 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M Calcium acetate, 0.1 M MES pH 6.0, 20% w/v Polyethylene glycol 8,000 |
