9KX0
Crystal structure of CAMP3 factor of Cutibacterium acnes.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-09-27 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.979183 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 88.325, 88.325, 98.557 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.200 - 2.430 |
| R-factor | 0.24612 |
| Rwork | 0.243 |
| R-free | 0.30108 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.277 |
| Data reduction software | autoPX |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0415) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.430 | 2.430 |
| Rmerge | 0.070 | 0.644 |
| Rmeas | 0.071 | 0.661 |
| Number of reflections | 15274 | 1102 |
| <I/σ(I)> | 56.34 | 5.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 24.15 | 19.91 |
| CC(1/2) | 1.000 | 0.957 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | 26%(w/v)PEG 3350 41 mM Citric acid 59 mM Sodium citrate |
