9KU0
Crystal structure of CtpA F105R mutant from Helicobacter pylori
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE TPS 05A |
| Synchrotron site | NSRRC |
| Beamline | TPS 05A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2022-07-04 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1.0000 |
| Spacegroup name | H 3 |
| Unit cell lengths | 139.331, 139.331, 252.092 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.010 - 3.700 |
| R-factor | 0.246 |
| Rwork | 0.242 |
| R-free | 0.31240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.872 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_5526: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.010 | 4.050 |
| High resolution limit [Å] | 3.700 | 3.700 |
| Rmerge | 0.126 | 0.521 |
| Number of reflections | 19454 | 4677 |
| <I/σ(I)> | 7.9 | |
| Completeness [%] | 99.8 | |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 100 mM Bis-tris (pH 6.5), 100 mM magnesium chloride, 25% PEG 3350 |
