9KSG

Crystal structure of RxLR145 effector

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-09
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	71.034, 89.587, 147.716
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.151 - 2.577
R-factor	0.2233
Rwork	0.219
R-free	0.28900
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.394
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	SOLVE
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.151	2.660
High resolution limit [Å]	2.577	2.577
Rmerge	0.101	0.464
Number of reflections	30135	30135
<I/σ(I)>	37.73
Completeness [%]	99.9
Redundancy	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		283	0.03M Citric acid, 0.07M BIS-TRIS propane pH 7.6, 20% w/v PEG 3350