9KRR
CRYSTAL STRUCTURE OF REDUCED CYTOCHROME C6 FROM Synechocystis PCC 6803
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-01-26 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.899, 74.437, 133.182 |
| Unit cell angles | 90.00, 106.11, 90.00 |
Refinement procedure
| Resolution | 56.390 - 1.930 |
| R-factor | 0.1691 |
| Rwork | 0.168 |
| R-free | 0.21020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.888 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.000 | 2.040 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.072 | 0.494 |
| Rpim | 0.036 | 0.262 |
| Number of reflections | 105090 | 15145 |
| <I/σ(I)> | 12.6 | 3 |
| Completeness [%] | 99.8 | |
| Redundancy | 5.6 | 5 |
| CC(1/2) | 0.998 | 0.845 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 0.2M Sodium iodide, 2.2M Ammonium sulfate |
