9KQH
Crystal structure of Finegoldia magna DinG in complex with ssDNA and ADPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2024-04-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979183 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 97.857, 123.534, 84.472 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.510 - 2.260 |
| R-factor | 0.2412 |
| Rwork | 0.240 |
| R-free | 0.27140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.498 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.470 | 2.380 |
| High resolution limit [Å] | 2.260 | 2.260 |
| Rmerge | 0.097 | 0.066 |
| Number of reflections | 46344 | 1694 |
| <I/σ(I)> | 10.2 | 16.2 |
| Completeness [%] | 95.4 | |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.2 M Ammonium citrate dibasic, 20% PEG3350 |
