9KQD

YcfA-D19A-ATP-GTP complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-08-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 1 2 1
Unit cell lengths	74.163, 135.089, 234.795
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.890 - 2.100
R-factor	0.16725
Rwork	0.166
R-free	0.19226
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	2.238
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.890	2.151
High resolution limit [Å]	2.100	2.100
Number of reflections	268269	25828
<I/σ(I)>	16.53
Completeness [%]	99.3
Redundancy	5
CC(1/2)	0.999	0.692

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	0.2 M sodium malonate pH 7.0 and 20% PEG3350