9KQ7
The structure of the YcfA from Erwinia amylovora bound with ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-08-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 69.710, 134.330, 235.960 |
| Unit cell angles | 90.00, 98.43, 90.00 |
Refinement procedure
| Resolution | 33.340 - 2.840 |
| R-factor | 0.21178 |
| Rwork | 0.210 |
| R-free | 0.25248 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.307 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.340 | 2.913 |
| High resolution limit [Å] | 2.840 | 2.840 |
| Number of reflections | 101379 | 10114 |
| <I/σ(I)> | 11.32 | |
| Completeness [%] | 99.8 | |
| Redundancy | 2 | |
| CC(1/2) | 0.996 | 0.542 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289.15 | 0.2 M sodium malonate pH 7.0 and 20% PEG3350 |
